Table 2 Name, function, primer sequences and amplicon characteristics of candidate reference genes (bp, base pairs; E, PCR efficiency)
Name | Accession No. | Function | Sequence (5‘ -> 3‘) | Amplicon (bp) | E (%) |
|---|---|---|---|---|---|
ACTB | NM_001101 | Cytoskeletal structural protein | F: AGA GCT ACG AGC TGC CTG AC R: AGC ACT GTG TTG GCG TAC AG | 184 | 92.48 |
B2M | NM_004048 | Beta-chain of MHC-I molecules | F: CAC TGA ATT CAC CCC CAC TGA R: CTG CTT ACA TGT CTC GAT CCC A | 104 | 92.94 |
GAPDH | NM_002046 | Enzyme of glycolysis | F: CCA TGA GAA GTA TGA CAA CAG CC R: GGG TGC TAA GCA GTT GGT G | 70 | 96.28 |
GUSB | NM_000181 | Exoglycosidase in lysosomes | F: ACT TCT CTG ACA ACC GAC GC R: AGG ATC ACC TCC CGT TCG TA | 172 | 95.21 |
HPRT | NM_000194 | Glycosyltransferase mutase | F: GAC CAG TCA ACA GGG GAC AT R: AAG CTT GCG ACC TTG ACC AT | 167 | 93.83 |
PPIB | NM_000942 | Cyclosporine-binding protein | F: GCC GGG TGA TCT TTG GTC TC R: AAG TCT CCG CCC TGG ATC AT | 148 | 100.55 |
RPLP0 | NM_001002 | Ribosomal protein | F: TGG CAG CAT CTA CAA CCC TG R: ATC TGC AGA CAG ACA CTG GC | 102 | 90.21 |
RPL13 | NM_012423 | Ribosomal protein | F: CGA GGT TGG CTG GAA GTA CC R: CTT CTC GGC CTG TTT CCG TAG | 121 | 92.44 |