Fig. 3

Absence of editing plasmid-specific fragments in genomic DNA extracted from the parental cell line (BEF2), the gene-edited cell clone CC14, DNA sent away for WGS of CC14 (CC14*), and genomic DNA extracted from cloned calves B071, 1805, and 1802. Each PCR reaction contained two sets of primers and BEF2 spiked in with 0.14pg of plasmid DNA was used as the positive control. (a) Primer pair designed to amplify bovine BTA2:110,817,757 − 110,818,275 bp (519 bp), and another designed to amplify CRISPR-Cas9 expression plasmid-specific region 6,263-7,019 bp (757 bp); (b) Primer pair designed to amplify bovine BTA2:110,817,757 − 110,818,275 bp (519 bp product), and another to amplify plasmid-specific region 6,939-7,628 bp (690 bp). (a) and (b) have been cropped, and full-length gels are presented in Supplementary Figure 2