Fig. 4
From: Stage-specific transcriptomic changes in pancreatic α-cells after massive β-cell loss
Epigenomic analyses reveal stage-specific marker genes potentially driving the α-cell response to β-cell loss. A Proportion of genes from each cluster associated with transcription factor binding sites, and proportion of cluster genes with their promoter interacting with at least 1 islet enhancer. B Expression profiles for selected cluster 1–5 markers. Codes below each gene name indicate association with at least one transcription factor binding site in human islets (&), promoter physical interaction (pcHi-C) with at least one human islet enhancer (¶) and whether the gene encodes for a transcription factor (#). NH: No human homolog found. * P < 0.05. C Violin plots showing the single-cell expression profiles for selected cluster marker genes in mouse pancreatic islet endocrine cell types (data from [28]). D Integrative map of the IFIT3 locus showing human islet ChIP-seq signal for 5 key pancreatic islet transcription factors, histone modification enrichment profiles associated with active (H3K27ac) promoter (H3K4me3) and enhancer (H3K4me1) regions, islet enhancer hubs, islet regulome regions and arcs representing high-confidence pcHi-C interactions in human islets (data from [18, 26])