Skip to main content
Fig. 1 | BMC Genomics

Fig. 1

From: Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors

Fig. 1

slc45a2 and dnd FLAG knock-in (1.5 μM ODN). a. Asymmetrical ODNs were designed by copying 90 + 36 nucleotides on each side of the CRISPR cut site flanking the insert (indicated with a dotted line) containing the FLAG element followed by a STOP codon (TAA). b. Relative read counts per individual for slc45a2 (red dots, n = 30) and dnd (blue dots, n = 24). Reads with a perfect match to the entire target sequence are referred to as perfect HDR. Reads with a correct insert flanked by mismatches/indels on the 5′ and/or 3′-side are referred to as erroneous HDR. Error bars indicate SEM/group

Back to article page