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Fig. 2 | BMC Genomics

Fig. 2

From: Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors

Fig. 2

slc45a2 FLAG knock-in. The asymmetrical ODN targeting slc45a2 was tested using three different concentrations: 0.5 (n = 23), 1.5 (n = 30) and 4.0 (n = 23) μM. Sequence reads with a perfect match to the entire target sequence are referred to as perfect HDR and reads with a correct insert but mismatches/indels in the homology arms are referred to as erroneous HDR. Read counts for each sample are given in % of the total number of reads. The error bars indicate SEM/group. Different lowercase letters indicate significant differences (P < 0.05), ns = non-significant

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