Fig. 3

Single nucleotide replacement in dnd. a. An asymmetrical ODN targeting dnd was designed with 90 + 36 nucleotides on each side of the CRISPR cut site and three nucleotides were changed. PAM site is shown with brown letters, and novel nucleotides with red letters. b. HDR rates for three different ODN concentrations; 0.15 (n = 24), 1.5 (n = 26) and 4.0 μM (n = 12). Sequence reads with a perfect match to the entire target sequence are referred to as perfect HDR (blue) and reads with a correct SNR but mismatches/indels in the homology arms are referred to as erroneous HDR (gray). Read counts for each sample are given in % of the total number of reads. Error bars indicate SEM/group. Different lowercase letters indicate significant differences (P < 0.05)