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Fig. 4 | BMC Genomics

Fig. 4

From: Single nucleotide replacement in the Atlantic salmon genome using CRISPR/Cas9 and asymmetrical oligonucleotide donors

Fig. 4

Variation in indel locations. Here, we distinguished between reads with a perfect match to the 5′- or 3′-side of the FLAG insert/SNR. The asymmetrical ODNs were compared at 1.5 μM. Green dots represent perfect 5′ reads and squares represent perfect 3′ reads. Read counts for each sample are given in % of the total number of reads. Error bars indicate SEM/group. The groups slc45a2 FLAG KI (n = 30), dnd FLAG KI (n = 19) and dnd SNR (n = 24) were analyzed separately. Different lowercase letters indicate significant differences (P < 0.05)

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