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Fig. 2 | BMC Genomics

Fig. 2

From: Insertion of an endogenous Jaagsiekte sheep retrovirus element into the BCO2 - gene abolishes its function and leads to yellow discoloration of adipose tissue in Norwegian Spælsau (Ovis aries)

Fig. 2

A 7.9 kb endogenous Jaagsiekte Sheep Retrovirus (enJSRV) element inserted into the first intron of the ovine BCO2 - gene. A The BCO2 first intron is shown together with exon 1 and exon 2. The insertion point of the enJSRV is indicated in red, 730 bp downstream of exon 1. The line below is showing the corresponding chromosome 15 positions in bp. B BCO2 intron 1 is shown with and without the inserted endogenous virus element. C Primer pairs for amplifying the enJSRV insertion point when no insertion is present (7552/7553), the upstream intron 1/enJSRV junction (7554/7555) and the downstream enJSRV/intron 1 juction (7556/7557). Distances are not drawn to scale. D Gel separations of PCR-products resulting from the primer combinations shown in the previous section (c). Genotypes are scored as wild type (wt) or carrier of the endogenous Jaagsiekte Sheep Retrovirus (ins). Example lanes are copied from 3 different gels used for genotyping (Fig. S2b,c,d). The primer combinations 7552/7553, 7554/7555 and 7556/7557 are shown from left to right. The lanes resulting from each of the 3 primer pairs are grouped together for the genotypes wt/wt, wt/ins and ins/ins, respectively. The size marker in lane 1 is 100 bp GeneRuler®. E The corresponding phenotypes of the genotypes shown in previous section (d). Photo: Nortura SA

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