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Table 1 Overview of the read assignment accuracy of EAGLE-RC against the concatenation method with real datasets. The first part of the table provides details on each dataset such as the species of origin, the type of replication (biological or technical), the sequencing strategy and the divergence between the two progenitor species, represented by the two-way average nuclear identity (ANI). The sequencing strategy includes the sequencing layout (PE = paired-end, SE = single-end) followed by the read length in bp. The two-way ANI was obtained using the ANI calculator from [56] with default parameters. The ANI value for Mimulus could not be calculated because of excessive computation time requirements (> 6′000 CPU hours). The second part of the table shows the average number of uniquely mapped reads for each approach, which was used to calculate the average error rate on the third part of the table. The error rate was obtained by the number of reads assigned to the wrong genome divided by the total number of reads that were uniquely mapped and deduplicated

From: ARPEGGIO: Automated Reproducible Polyploid EpiGenetic GuIdance workflOw

Datasets

Average number of uniquely mapped reads

Average error rate

Species

Type of replicate (#)

Sequencing layout and read length

Two-way average nuclear identity

Concatenated genome

Read classification

Concatenated genome

Read classification

Arabidopsis halleri

Biological (2)

PE150

94.29 ± 3.94%

17′258’758

18′311’330

3.98%

1.16%

Arabidopsis lyrata

Biological (2)

PE150

22′204’342

23′301’056

5.94%

1.45%

Mimulus guttatus

Technical (4)

SE150

N.A.

1′420’116

1′288’800

26.78%

7.52%

Mimulus luteus

Technical (4)

SE150

3′889’458

3′760’614

9.80%

2.29%

Gossypium arboreum

Technical (2)

PE125

91.07 ± 4.68%

253′912’667

254′261’702

0.0044%

0.0013%

Gossipyium raimondii

Technical (2)

PE125

242′590’069

246′935’598

0.0039%

0.0019%