Fig. 2

Alignment of the extracellular juxtamembrane, transmembrane and cytoplasmic region of the RLPs. Most of the prRLPs (boxed in yellow) have both motifs required for interaction with SOBIR1, the negatively charged amino acid stretch in the eJM and the GxxxG-motif in the TM, except RLP28 lacking both motifs and RLP46 having only two Gs. Most of the bRLPs (boxed in blue) lack the GxxxG-motif, except RLP44 and RLP57, but the latter lack a dominant negatively-charged amino acid stretch in the eJM. All sequences were aligned using MUSCLE and the sequences were ordered manually to fit the phylogenetic tree. The last Leucine rich repeat-domain (LRR), as well as the extracellular juxtamembrane (eJM), the transmembrane domain (TM) and the cellular juxtamembrane (cJM) are indicated. Color coded in magenta are the Glycines (G) in the TM and in cyan the Aspartates (D) and Glutamates (E) in the eJM