Fig. 4

Frequency distribution of k-mers (k = 21 bp) in NGS reads from pooled S. nebaliae DNAs. According to GenomeScope, distributions were 73.6% identical between the processed NGS data and the diploid genome model. Observed k-mers left of the red line (light blue fill) were classified as errors due to their low frequencies but will also include k-mers from less abundant carriers of nuclear genome A. Blue fill right of the red line should correspond to k-mers derived of S. nebaliae specimens carrying nuclear genome B. The shift in multiplicity between observation (77 x) and the heterozygous peak in the diploid model (60 x) is likely due to genetic variation in lineage B. GenomeScope v. 1.0 [42] was run with trimmed and filtered DNA reads