Frequent tRNA gene translocation towards the boundaries with control regions contributes to the highly dynamic mitochondrial genome organization of the parasitic lice of mammals

Table 4 Statistical analysis of expected probability and observed probability of tRNA gene translocation in three Polyplax species relative to their most recent common ancestor

tRNA genes	Expected probability of translocation to be adjacent to NCR if translocation is random	Observed probability of translocation to be adjacent to NCR	Expected probability of translocation to an intergenic region if translocation is random	Observed probability of translocation to an intergenic region
A	43.75 % (21/48)	100 % (2/2)	56.25 % (27/48)	0 %
F	43.75 % (21/48)	100 % (1/1)	56.25 % (27/48)	0 %
P	45.83 % (22/48)	100 % (1/1)	54.12 % (26/48)	0 %
T	45.83 % (22/48)	100 % (2/2)	54.12 % (26/48)	0 %
S₁-S₂	44.68 % (21/47)	100 % (3/3)	55.32 % (26/47)	0 %
Average	44.8 %	100 %	55.2 %	0 %
Two tailed t-value	-118.61978		115.73698
p-value	< 0.00001		< 0.00001
Significant at p < 0.01?	Yes		Yes

Notes: Refer to Table 3 for tRNA gene translocation in Polyplax species. “Expected probability of translocation to be adjacent to NCR” = “total sites adjacent to NCR” / “total sites adjacent to NCR + total intergenic sites”. “Expected probability of translocation to an intergenic region” = “total intergenic sites” / “total sites adjacent to NCR + total intergenic sites”. “Observed probability of translocation to be adjacent to NCR” = “translocation adjacent to NCR” / “translocation adjacent to NCR + translocation to intergenic sites”. “Observed probability of translocation to an intergenic region” = “translocation to intergenic sites” / “translocation adjacent to NCR + translocation to intergenic sites”. T-Test was done at: https://www.socscistatistics.com/tests/studentttest/default2.aspx

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