Frequent tRNA gene translocation towards the boundaries with control regions contributes to the highly dynamic mitochondrial genome organization of the parasitic lice of mammals

Table 6 Statistical analysis of expected probability and observed probability of tRNA gene translocation in the most recent common ancestor (MRCA) of Polyplax lice relative to the MRCA of sucking lice

tRNA genes	Expected probability of translocation to be adjacent to NCR if translocation is random	Observed probability of translocation to be adjacent to NCR	Expected probability of translocation to an intergenic region if translocation is random	Observed probability of translocation to an intergenic region
N	21/46 = 45.65 %	100 %	25/46 = 54.35 %	0 %
S₁-S₂	21/45 = 46.67 %	100 %	24/45 = 53.33 %	0 %
I	21/46 = 45.65 %	100 %	25/46 = 54.35 %	0 %
Q	21/46 = 45.65 %	100 %	25/46 = 54.35 %	0 %
Average	45.9 %	100 %	54.1 %	0 %
Two tailed t-value	-212.13726		212.13726
p-value	< 0.00001		< 0.00001
Significant at p < 0.01?	Yes		Yes

Notes: Refer to Table 5 for tRNA gene translocation in the MRCA of Polyplax species. Refer to Table 4 notes for probability calculation and T-Test

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