Frequent tRNA gene translocation towards the boundaries with control regions contributes to the highly dynamic mitochondrial genome organization of the parasitic lice of mammals

Table 8 Statistical analysis of expected probability and observed probability of tRNA gene translocation in non-Polyplax sucking lice relative to the most recent common ancestor of sucking lice

tRNA genes	Expected probability of translocation to be adjacent to NCR if translocation is random	Observed probability of translocation to be adjacent to NCR	Expected probability of translocation to an intergenic region if translocation is random	Observed probability of translocation to an intergenic region
N	21/45 = 46.67 %	20 %	24/45=53.33%	80 %
E	21/45 = 46.67 %	33.3 %	24/45 = 53.33 %	66.7 %
S₁	22/46 = 47.83 %	50 %	23/46 = 52.17 %	50 %
S₂	21/45 = 46.67 %	50 %	24/45 = 53.33 %	50 %
I	21/45 = 46.67 %	100 %	24/45 = 53.33 %	0 %
L₂	21/45 = 46.67 %	100 %	24/45 = 53.33 %	0 %
D	21/45 = 46.67 %	25 %	24/45 = 53.33 %	75 %
P	22/46 = 47.83 %	100 %	23/46 = 52.17 %	0 %
A	21/45 = 46.67 %	0 %	24/45 = 53.33 %	100 %
Q	21/45 = 46.67 %	33.3 %	24/45 = 53.33 %	66.7 %
T	22/46 = 47.83 %	100 %	23/46 = 52.17 %	0 %
G	22/46 = 47.83 %	100 %	23/46 = 52.17 %	0 %
W	21/45 = 46.67 %	0 %	24/45 = 53.33 %	100 %
H	21/45 = 46.67 %	50 %	24/45 = 53.33 %	50 %
F	21/45 = 46.67 %	33.3 %	24/45 = 53.33 %	66.7 %
C	21/45 = 46.67 %	100 %	24/45 = 53.33 %	0 %
M	21/45 = 46.67 %	80 %	24/45 = 53.33 %	20 %
L₁	22/46 = 47.83 %	100 %	23/46 = 52.17 %	0 %
V	21/45 = 46.67 %	75 %	24/45 = 53.33 %	25 %
Average	46.9 %	60.5 %	53.1 %	39.5 %
Two tailed t-value	-1.60738		1.61464
p-value	0.116709		0.11512
Significant at p < 0.01?	No		No

Notes: Refer to Table 7 for tRNA gene translocation in non-Polyplax sucking lice. Refer to Table 4 notes for probability calculation and T-Test

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