Fig. 8

Decrease of Phred score in ONT reads when running from genomic sequence into a T-DNA array. The insertion used as example is from line GK-038B07 and is shown in Fig. 1 C. A/B: Read quality data from two ONT reads with opposite direction. The reads are displayed in 5’ to 3’ orientation. Green and blue bars indicate the read parts matching Chr3 and Chr5, respectively. The red bar indicates the position of the T-DNA array which is arranged as inverted repeat. Read IDs of the two examples are shown above each panel. The read quality drop to a Phred score of about 7 occurs when the second part of the inverted repeat is sequenced