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Fig. 3 | BMC Genomics

Fig. 3

From: circ_0038718 promotes colon cancer cell malignant progression via the miR-195-5p/Axin2 signaling axis and also effect Wnt/β-catenin signal pathway

Fig. 3

miR-195-5p is a direct target of and negatively regulated by circ_0038718. (A) 21 DE_miRNAs obtained from the GSE126093 Series Matrix File; (B) 12 down-regulated DE_miRNAs were intersected with 115 predicted target genes of circ_0038718; (C) Relative expression of miR-195-5p in GSE126093 Series Matrix File. Blue and red boxes stand for the normal and tumor groups, respectively; (D) Predicted binding site between miR-195-5p and circ_0038718; (E) qRT-PCR was conducted to test the expression of circ_0038718, U6 and GAPDH in cytoplasm and nucleus after nucleocytoplasmic separation of HT-29 and SW480 cells; (F-G) Dual-luciferase reporter assay and RIP experiment were performed to verify the targeting relationship between miR-195-5p and circ_0038718; (H) qRT-PCR was used to evaluate the effect of circ_0038718 over-expression on miR-195-5p; (I) The expression of miR-195-5p in colorectal cancer tissue and adjacent tissue, respectively; (J) The correlation between circ_0038718 and miR-195-5p expression level. * p < 0.05

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