Fig. 5

miR-195-5p regulates Wnt/β-catenin signaling pathway by targeting Axin2. (A) Volcano plot of 415 DE_mRNAs in the GEO database (Red and green dots represent up-regulated and down-regulated genes, respectively); (B) Venn diagram of up-regulated DE_mRNAs in the GEO database and the predicted target mRNAs of miR-195-5p; (C) Relative expression of Axin2 in the GEO database (Blue and red boxes represent normal and tumor group, respectively); (D) The expression level of Axin2 in colorectal cancer tissue and adjaceent tissue; (E) The correlation between Axin2 and miR-195-5p expression level; (F) The correlation between Axin2 and circ_0038718 expression level; (G) Putative binding sites between miR-195-5p and Axin2; (H) Dual-luciferase reporter assay was performed to detect the targeting relationship between miR-195-5p and Axin2; (I) qRT-PCR was performed to assess the effect of over-expressing miR-195-5p on Axin2 mRNA; (J) Western blot was conducted to evaluate the effect of miR-195-5p over-expression on the protein expression of Axin, β-catenin, c-Myc and cyclin D1; (K) qRT-PCR was performed to assess the effect of sh-Axin2 knock down. * p < 0.05