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Fig. 7 | BMC Genomics

Fig. 7

From: Deploying new generation sequencing for the study of flesh color depletion in Atlantic Salmon (Salmo salar)

Fig. 7

Comparison of variations within biological replicates of 15 DEGs from the Quantseq and Trueseq libraries and RT-qPCR validation using the same biological samples; Gene expression in (A) QuantSeq library; (B) TruSeq library; (C) RT-qPCR. From left to right, the first five genes were significantly different between HN and LB in both library methods but only ldtn shows a significant difference in RT-qPCR assay; the next five genes showed significant differences only in the QuantSeq library analysis, and only two of those (fabp1 and lyama) were significantly different in RT-qPCR; the last five genes showed significant differences only in the TruSeq library, and only three out of these five genes (ovcm2, noxo1 and fclt3) were significantly different using the RT-qPCR assay. Asterisks (* and **) indicate significant difference between the HN and LB phenotypes at P < 0.05 and P < 0.01, respectively

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BMC Genomics

ISSN: 1471-2164

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