Table. 2 Sixteen DEGs selected for validation with RT-qPCR
Gene ID | DEG Group | Forward primer 5’-3’ | Reverse primer 5’-3’ |
|---|---|---|---|
(mfi2) antigen p97 (melanoma associated) | QuantSeq only | cagcagggaaaagctacgg | tcactgtcccctgtgtagctc |
(epdm) ependymin-like | QuantSeq only | aaccacacaatgcaagccta | agctcccaacagccagatac |
(lyama) lysosomal alpha-mannosidase | QuantSeq only | aacagctacctgcagacgtg | tttccttgagatgggaccac |
(fabp1) fatty acid binding protein 1, liver | QuantSeq only | gggcaccaaggtcatagtca | tcttctctccagtcaaagtctcg |
(fertn-m) ferritin, middle subunit-like | QuantSeq only | cgtgatgagtggggcaat | cagggcctggttcacatt |
(s2p4a-l) SH2 domain-containing protein 4Â A-like | TruSeq only | gcaacagcacagacgaccta | atgttgctgtggtgggttct |
(noxo1) NADPH oxidase organizer 1-like | TruSeq only | atgggctggaggacatga | gctcacaaaagggtgtgtca |
(fclt3) fucolectin-3-like | TruSeq only | atgagggcattcacaacaca | tccttccacacctgatgtcc |
(tetn) tetranectin | TruSeq only | gtcagtggtgtgcgtttgtt | tctgttggaatgtggattgc |
(ovcm2) ovochymase-2-like | TruSeq only | cgtttcctcagcaaccaag | gggatcggtggtccagtaa |
(ldltn) ladderlectin-like | Both libraries | ctttgtgtcgccctctctg | gacattgttgagtatttggctgtc |
(cd209) CD209 antigen-like | Both libraries | tggtcacaattaaataatgttttgg | gaatgtttatttagtcatgctggtg |
(es1p) ES1 protein homolog, mitochondrial-like | Both libraries | agttgacttccagttacactacaacag | gcagtctgggtcgtcttctc |
(umy1b) unconventional myosin-Ib-like | Both libraries | aggaatgccatgcagattgt | accagctccagaaccgact |
(glfsy) GDP-L-fucose synthase | Both libraries | ctgattggctgtcaagcaac | gaaggtttgactccccatga |
(18Â S) 18Â S ribosomal RNA | House keeping gene | aggactccggttctattttgtg | cggccgtccctcttaatc |