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Fig. 8 | BMC Genomics

Fig. 8

From: Putative genes in alkaloid biosynthesis identified in Dendrobium officinale by correlating the contents of major bioactive metabolites with genes expression between Protocorm-like bodies and leaves

Fig. 8

RT-qPCR analysis and number of differentially expressed transcription factors. RT-qPCR analysis of genes encoding enzymes involved in alkaloid biosynthesis (a), polysaccharide biosynthesis (b), and flavonoid biosynthesis (c). The number of major transcription factor genes up-regulation and down-regulation of in PLBs comparing to leaves of D. officinale (d). Subunit 5.8S of rRNA was used as an internal gene for normalization. PLB and leaf samples were used as normalizers in each experiment. Blue bars represent RT-qPCR data, and red lines indicate FPKM values from RNA-seq. Data represent mean ± standard error of three replicates. The left Y-axis denotes relative expression levels of genes determined by RT-qPCR, and the right Y-axis denotes the FPKM values of RNA-Seq data

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