Table 2 The effects of different factors on PLBs proliferation
Levels | Â | Factors | Propagation coefficient | |||
|---|---|---|---|---|---|---|
Media (A) | α-NAA (B) mg·L−1 | 6-BA (C) mg·L− 1 | 2,4-D (D) mg·L− 1 | Potato juice (E) g·L− 1 | ||
1 | 1/4MS | 0.1 | 0.5 | 1.5 | 50.0 | 2.14 |
2 | 1/4MS | 0.2 | 1.0 | 2.0 | 100.0 | 3.98 |
3 | 1/4MS | 0.5 | 1.5 | 2.5 | 150.0 | 3.23 |
4 | 1/4MS | 1.0 | 2.0 | 3.0 | 200.0 | 1.98 |
5 | 1/2MS | 0.1 | 1.0 | 2.5 | 200.0 | 4.54 |
6 | 1/2MS | 0.2 | 0.5 | 3.0 | 150.0 | 1.83 |
7 | 1/2MS | 0.5 | 2.0 | 1.5 | 100.0 | 4.57 |
8 | 1/2MS | 1.0 | 1.5 | 2.0 | 50.0 | 3.11 |
9 | MS | 0.1 | 1.5 | 3.0 | 100.0 | 3.42 |
10 | MS | 0.2 | 2.0 | 2.5 | 50.0 | 2.75 |
11 | MS | 0.5 | 0.5 | 2.0 | 200.0 | 4.63 |
12 | MS | 1.0 | 1.0 | 1.5 | 150.0 | 5.02 |
13 | 2MS | 0.1 | 2.0 | 2.0 | 150.0 | 2.65 |
14 | 2MS | 0.2 | 1.5 | 1.5 | 200.0 | 1.93 |
15 | 2MS | 0.5 | 1.0 | 3.0 | 50.0 | 4.87 |
16 | 2MS | 1.0 | 0.5 | 2.5 | 100.0 | 2.53 |
K1 | 2.83 | 3.19 | 2.78 | 3.60 | 3.22 | Â |
K2 | 3.51 | 2.62 | 4.60 | 3.59 | 3.62 | Â |
K3 | 3.96 | 4.32 | 2.92 | 3.26 | 3.18 | Â |
K4 | 3.00 | 3.16 | 2.99 | 3.02 | 3.27 | Â |
R | 1.13 | 1.16 | 1.82 | 0.52 | 0.44 | Â |