Fig. 3
Distribution of differentially methylated m6A sites (DMMSs). A Chromosomal distribution of all DMMSs within mRNAs. B Relative occupancy of DMMSs in each chromosome. C Data visualization analysis of two representative hyper- and hypo-methylated genes in Fts and Fs groups. The green rectangles show the locations of differentially methylated peaks. D The validation of total m6A methylation level of ten mRNAs with differentially methylated sites selected from MeRIP-seq data. Fs, fresh sperm; Fts, frozen-thawed sperm. “*P < 0.05, **P < 0.01”