Table 1 Functions of the differentially expressed and methylated DEGs between Fs and Fts during cryopreservation
Gene symbol | Regulation (Fts vs. Fs) | Description | |||||
|---|---|---|---|---|---|---|---|
Methylation | Fold Change | p_value | mRNA expression | Fold Change | p_value | ||
ACLY | Up | 2.8 | 5.18E-06 | Down | -inf | 2.72e-02 | Regulation of the ram sperm energy metabolism and ATP supplement [34, 35] |
PDE4A | Up | 2.8 | 4.67E-06 | Up | 2.3 | 3.14e-02 | Regulation of bovine sperm motility [36] |
NFATC3 | Up | 11.3 | 3.20E-06 | Down | -inf | 7.20e-03 | Differentially represented between fresh- and frozen-sperm in boar [31] |
BCL2L1 | Up | 3.1 | 8.07E-06 | Down | -inf | 2.80e-03 | Cell apoptosis protecting [37] |
CARD6 | Up | 2.9 | 4.07E-06 | Down | -inf | 5.00e-05 | Regulation of apoptosis [38] |
PIK3R1 | Up | 3.2 | 2.56E-06 | Down | -inf | 5.00e-05 | Involved in actin polymerization during bovine sperm capacitation [39] |
NCALD | Up | 2.3 | 1.13E-06 | Down | -inf | 5.00e-05 | Regulation of Calcium-modulated transduction in human and bovine sperm [40] |
SOX9 | Up | 11.0 | 8.73E-07 | Down | -inf | 5.00e-05 | Regulate male fertility in mice [41] |
PLCB1 | Up | 7.7 | 5.65E-06 | Down | -inf | 9.50e-04 | Essential for acrosome reaction and fertilization in mouse sperm [42] |