Table 3 cif genes in Wolbachia strains of Rhagoletis cerasi

wCer1

INQ25_05555

491

cifA

wHa_RS01435, 492, 91%

I

UNCERTAIN: No crossing experiment data exists; but strong linkage with mitochondrial haplotype (HT1) in Rhagoletis cerasi field populations

INQ25_05550

1143

cifB

wHa_RS01430, 1148, 91%

I

INQ25_01115

491

cifA

wHa_RS01435, 492,88%

I

INQ25_01120

1150

cifB

wHa_RS01430, 1148, 92%

I

wCer2

E3V96_03425

475

cifA

wMel_RS02835, 475, 99%

I

YES: Experimental evidence for CI in multiple novel hosts - Drosophila simulans, Ceratitis capitata, Bactrocera oleae; strong linkage of wCer2 with mitochondrial haplotype (HT2) in R. cerasi field populations

E3V96_03430

1174

cifB

wMel_RS06940, 1174, 99.7%

I

E3V96_02935

481

cifA

wVitA_RS00555, 499, 75%

I

E3V96_02940

1531

cifB

wVitA_RS00550, 1523, 85%

I

E3V96_06520

492

cifA

wPip_RS01410, 504, 90%

I

E3V96_06515

921

cifB

wPip_RS01415, 1175, 83%

I

E3V96_03725

438

cifA

wIrr_E0495_RS03300, 429, 66%

V

E3V96_03720

3405

cifB

wStriCN_BVG17_RS00730, 3083, 72%

V

wCer4

INQ27_01280

494

cifA

wHa_RS01435, 492, 79%

I

YES: Experimental evidence for strong CI in novel host C. capitata

INQ27_01275

1166

cifB

wPip_RS01415, 1175, 85%

I

INQ27_01270

415

cifA

wStriCN_BVG17_RS06595, 415, 84%

V

INQ27_01265

3332

cifB

wStriCN_BVG17_RS06590, 4358, 72%

V

wCer5

INQ21_01080

446

cifA

wPip_RS01460, 446, 99.8%

IV

UNCERTAIN: No crossing experiment data exists; but very high similarity of cif gene sequences to the Type IV cifA/B genes of wPip with proven CI induction

INQ21_01085

733

cifB

wPip_RS01465, 733, 99.6%

IV