Fig. 4
From: Reverse complementary matches simultaneously promote both back-splicing and exon-skipping
RCMs promote both back-splicing and exon-skipping. a Cropped gel image showing RT-PCR detection of full-length transcripts and skipped transcripts in zip-2 and Y20F4.4. b RT-qPCR quantification of Y20F4.4 transcripts in wild-type and RCM-deleted Y20F4.4 strain. c RT-qPCR quantification of arl-13 transcripts in wild-type and RCM-deleted arl-13 strain. d Illustration of the gene structure of zip-2. P1-P6: positions of primers. Black rectangles indicate coding regions and white parts are untranslated regions (UTRs). RCM areas are in red. e. Cropped gel image of RT-PCR detection of transcripts from zip-2 gene in wild-type N2 strain and RCM-deleted strains. f, g. RT-qPCR quantification of zip-2 transcripts in RCM-deleted strains compared with wild-type N2 strain. b, c, f, and g. Results are normalized to levels in N2 strain using pmp-3 as the reference gene. Error bars are the standard deviations of three biological replicates. ***, p < 0.001, ****, p < 0.0001, two-tail Student’s t-test