Table 2 Statistics of small RNA sequences in pollen-treated and control honey bees for detection of diet-derived sunflower and Sedar miRNAs

Raw reads

2,660,260

4,251,505

2,030,142

3,975,267

2,237,955

2,238,200

Clean reads

2,558,916

4,093,244

1,696,697

3,449,599

2,077,808

2,172,285

rRNA

1,096,486

1,336,965

642,603

1,079,158

439,323

681,660

tRNA

68,118

108,148

144,150

319,453

187,744

169,690

snRNA

40,226

27,042

16,751

39,478

14,056

15,065

snoRNA

5071

4814

2920

6974

2945

3504

Law complexity reads

4176

10,068

1414

3269

1263

1991

Contaminated reads

938

4651

505

850

623

598

Reads related to Apis mellifera miRNAs

111,446

573,125

65,904

140,928

79,284

124,621

Reads related to other insect miRNAs

3097

21,525

1483

2899

1413

1685

No annotation reads

801,690

1,202,178

623,295

1,421,167

1,071,775

887,345

Reads after removal of other small RNAs and all insect miRNAs

801,690

1,202,178

623,295

1,421,167

1,071,775

887,345

Reads mapped against Apis mellifera genome

410,890

735,370

226,332

543,661

298,952

289,856

Reads unmapped against Apis mellifera genome

390,800

466,808

396,963

877,506

772,823

597,489

Aligned reads to SF_miRNAs

56

73

4520

10,632

–

–

Aligned reads to Zizi_miRNAs

45

69

–

–

5670

4100

Count of the detected miRNAs of SF

0

0

11

31

–

–

Count of the detected miRNAs of Zizi

0

0

–

–

15

11