Fig. 1

Comparison of empirical and in silico restriction enzyme digestions. Empirical Bioanalyzer results with digested DNA are shown as concentration over fragment size (a, b, d, e, g, h) and estimated loci numbers over locus size from in silico digestions (c, f, i). The tests were conducted with restrictions enzymes EcoRI (a, b, c), ApeKI (d, e, f) and a double digest with EcoRI and MspI (g, h, i). Results for the fish species Trematomus bernacchii (a, d, g) and T. loennbergii (b, e, h) are shown next to in silico estimates using a related reference genome of Notothenia coriiceps and two simulated genomes of 100 and 180 Mb size (note that this was the absolute size used for in silico computations, but resulting estimates were extrapolated to 1000 and 1800 Mb). Results for other target taxa are shown in Additional File 3