Fig. 4
Prediction and filtering of the intersection of transcripts putatively differentially expressed among susceptible Diabrotica virgifera virgifera larvae exposed within independent treatments to maize roots expressing different Bacillus thuringiensis (Bt) pesticidal proteins. A) Correlation between estimated Log2(fold-change) among differentially-expressed transcripts from DESeq2 and EdgeR (FDR ≤ 0.05) between larvae exposed to non-Bt control hybrid maize roots (T7) with maize roots that express Bt Cry3Bb1 (T8; VT TriplePro® (VT3) hybrid) and Gpp34/Tpp35Ab1 (T5; Herculex® (Hx) hybrid). Venn diagram indicates the number of predicted differentially-expressed transcripts by DESeq2 and EdgeR surpassing significance thresholds (FDR ≤ 0.05), where the intersections (green up-regulated; red down-regulated) indicate those significant within both analyses. B) Comparison of differentially expressed transcripts between Bt and entomopathogen treatments, Heterorhabditis bacteriophora (Hb) and Metarhizium anisopliae (Ma). Differentially expressed transcripts within the intersection of those shared by both Bt and entomopathogen treatments, that were subtracted to arrive at final filtered set shared transcripts uniquely differentially expressed in both Cry3Bb1 (T8; VT3) and Gpp34/Tpp35Ab1 (T5; Hx) maize root treatments (green up-regulated; red down-regulated)