Up-regulation of apoptotic- and cell survival-related gene pathways following exposures of western corn rootworm to B. thuringiensis crystalline pesticidal proteins in transgenic maize roots

Table 7 Differential expressed apoptotic and autophagy-related protein-encoding transcripts

		Log₂(fold-change)
Transcript	Putative annotation	T7-T8	T7-T5	T7-T6	T7-T3
DIAVI022989	Initiator caspase-like (orthologs: STRICA or DAMM)	+ 3.21	+ 3.05	ns	ns
DIAVI027204	Effector caspase-like (ortholog: DECAY)	+ 3.00	+ 2.47	ns	ns
DIAVI057195	Stress-associated endoplasmic reticulum protein 2	+ 2.26	+ 2.13	ns	ns
DIAVI029891	Lifeguard 4-like (BAX inhibitor domain protein)	+ 2.02	+ 1.76	ns	ns
DIAVI013501	Autophagy-related gene 13 (atg13)-encoded protein	+ 2.20	+ 1.71	−1.42	−1.52
DIAVI011561	Programmed cell death protein 4	ns	+ 1.67	ns	ns
DIAVI026079	BAX inhibitor 1 (BI-1)	ns	+ 1.52	ns	ns
DIAVI011972	Inhibitor of apoptosis 1 (IAP1) isoform X1	ns	+ 2.22	ns	ns
DIAVI007715	Inhibitor of apoptosis 1 (IAP1) isoform X5	+ 4.36	ns	ns	ns

Response among susceptible Diabrotica virgifera virgifera Ped12 larvae exposed to Bacillus thuringiensis (Bt) maize roots and entomopathogens. Fold-change and direction (+ or -) of differential expression for transcripts is indicated for treatments with exposures to maize roots that express Bt pesticidal proteins Cry3Bb1 (T8; VT TriplePro® (VT3) hybrid)) and Gpp34/Tpp35Ab1 (T5; Herculex® (Hx) hybrid), and to the entomopathogens Heterorhabditis bacteriophora (T6) and Metarhizium anisopliae (T3), compared to non-Bt control (T7). Transcripts differentially expressed in the intersection of both Bt maize treatments are highlighted in gray. # Putative homologs from protein models in RefSeq accession GCF_003013835.1 annotated from genome assembly accession GCA_003013835.2 under the BioProject PRJNA432972: DIAVI022989 = XP_028132548.1; DIAVI027204 = XP_028144295.1; DIAVI057195 = XP_028135864.1; DIAVI029891 = XP_028155171.1; DIAVI013501 = XP_028130453.1; DIAVI011561 = XP_028128855.1 and XP_028128856.1; DIAVI026079 = XP_028141533.1; DIAVI011972 and DIAVI007715 = XP_028141650.1 to 58.1

ISSN: 1471-2164