Fig. 6

Conjoint analysis of proteome and acetylome data and identification of common regulated/modified proteins (DEPs and DAPs). A Volcano plot displaying all quantified differential acetylated proteins in Norm and FL group livers based on the acetylome data. Orange dots represent statistically significant up-regulated proteins (122) and blue dots represent down-regulated proteins (213). B Venn diagram displaying 54 overlapping protein that are both significantly regulated in expression abundance and acetylation modified based on proteome and acetylome, respectively. C Venn diagram displaying the distribution of the 54 proteins in regulation (P-up and/or P-down) and modification (Ac-up and/or Ac-down) direction. D Heat-map showing the subcellular localization of the 54 overlapping proteins according to the Uniprot database annotation. E and (F)Volcano plot displaying the expression level (E) and the acetylation level (F) of the 54 overlapping proteins found in proteome and acetylation data, respectively. G Protein-protein interaction (PPI) analysis of the overlapping 54 proteins. Circle size represents the interaction intensity of proteins. Circle color represents the regulation direction, up-regulated (Fold change > 1.2, P < 0.05) proteins in red and down-regulated (Fold change < 1/1.2, P < 0.05) proteins in green. Square color represents the significance of enriched pathways