Fig. 8

Genes that encode seminal vesicle secreted proteins are dysregulated following acrylamide exposure. A Expression of seminal vesicle secreted genes (n = 4 individual mice per treatment group) that were identified as statistically significant (adjusted p (p.adj) ≤ 0.1 indicated by *) in our transcriptomic analysis. Genes assessed included: Complement factor b (Cfb), Seminal vesicle secretory protein (SVS)2, SVS5, SVS4, and Deoxyribonuclease II beta (Dnase2b). Data are presented as column graphs of log fold-change (logFC) with the dashed line indicating the logFC threshold used to identify differentially regulated genes (logFC ≥0.58 or ≤ − 0.58). B RNA-sequencing data was validated using quantitative PCR on Cfb (n = 5 individual mice per treatment group). Data was processed using the delta C(t) method using Peptidyl-prolyl cis-trans isomerase A (Ppia) as the reference gene and then normalised to the control group. Data are presented as a column graphs with mean ± SEM values. Corresponding transcriptomics data is overlaid onto these data using a line graph. Data were analysed by non-parametric Mann Whitney U test. * p ≤ 0.05 indicates statistical significance when comparing acrylamide to control