Comparative analysis of the complete mitochondrial genome sequences and anther development cytology between maintainer and Ogura-type cytoplasm male-sterile cabbage (B. oleracea Var. capitata)

Table 1 The specific open reading frames (ORFs) in the Ogura CMS mitochondrial genome

Specific open-reading frames (orf)	the most similar mitochondrial sequence of other species	location in the Ogura CMS line
orf102a	YP_717109.1 hypothetical protein BrnapMp010 [Brassica napus]	In the Ogura-CMS Unique region II
orf122b	AEX57663.1 hypothetical protein RasatMp032 [Raphanus sativus]	In the Ogura-CMS Unique region II
orf138a	YP_006665998.1 hypothetical protein [Raphanus sativus]	In the Ogura-CMS Unique region I
orf154a	AEX57662.1 hypothetical protein RasatMp031 [Raphanus sativus]	Between Ogura-CMS block 4 and Unique region II

Four ORFs, including ORF102a, ORF122b, ORF138a, and ORF154a, were specifically identified in the Ogura CMS mitochondrial genome. ORF102a is closest to the mitochondrial protein of Brassica napus. ORF122b, ORF138a and ORF154a were most similar to the mitochondrial sequence of Raphanus sativus. ORF102a and ORF122b were located in the Ogura CMS unique region II and ORF138a was located at the left edge of the Ogura CMS unique region I; however, ORF154a was located between Ogura CMS block 4 and unique region II

ISSN: 1471-2164