Fig. 1

CPL1 regulates flowering time in Arabidopsis. a The structure of the CPL1 coding region. Black boxes, gray boxes and black lines represent exons, untranslated regions and introns, respectively. The point mutation is shown below. Red arrowheads indicate the positions of the primers used in Fig. 1b. b A cropped gel of the CAPS analysis of wild-type and the cpl1-3 mutant. Genomic DNA of wild-type (WT) and the cpl1-3 mutant were amplified using CAPS markers listed in Additional file 1, and then, the PCR products were digested with Sac I. c cpl1-3 mutant shows delay-flowering phenotype under long-day conditions. Scale bar: 2 cm. d and e Rosette leaf numbers (d) and days to bolting (e) of the cpl1-3 mutant grown under long-day conditions. f and g Rosette leaf numbers (f) and days to bolting (g) of the cpl1-3 mutant grown under short-day conditions. Values are representative of at least 15 plants showing specific genotypes. Asterisks indicate significant differences between WT and the cpl1-3 mutant in flowering time (Student’s t test, P < 0.05). h cpl1-1-3 35 S:CPL1-3FLAG exhibited a flowering time comparable to that of WT plants under long-day conditions. Scale bar: 2 cm. i and j Rosette leaf numbers (i) and days to bolting (j) of cpl1-1-3 35 S:CPL1-3FLAG grown under long-day conditions. Values are representative of at least 15 plants showing specific genotypes. Asterisks indicate significant differences between WT and the cpl1-3 mutant in flowering time (Student’s t test, P < 0.05)