Table 1 Analysis of the average of 20 genes in 4 indicators per method
From: Evaluation validation of a qPCR curve analysis method and conventional approaches
Methods | Bias(10000) | Bias(1) | Relative error | Coefficient of variation | Precision | Resolution |
|---|---|---|---|---|---|---|
CqMAN | 2469.0003 | 0.0182 | 0.2416 | 7.20% | 0.0020 | 1.9222 |
Cy0 | 2594.5631 | 0.0000 | 0.1050 | 9.62% | 0.0064 | 1.9213 |
LinRegPCR | 3891.3018 | 0.0523 | 0.3117 | 9.64% | 0.0064 | 1.9051 |
PCR Miner | 6167.5939 | 0.0561 | 0.2287 | 9.63% | 0.0062 | 1.9156 |
5PSM | 6041.4519 | 0.0999 | 0.6939 | 12.89% | 0.0096 | 3.0166 |
DART | 5233.9256 | 0.0840 | 0.5727 | 17.98% | 0.0140 | 2.8630 |
FPLM | 4814.3920 | 0.0745 | 0.5357 | 21.34% | 0.0182 | 3.1956 |
FPK-PCR | 8536.6094 | 0.0669 | 0.3592 | 25.12% | 0.0339 | 5.8404 |