From: Evaluation validation of a qPCR curve analysis method and conventional approaches
Methods | Bias(10,000/0.001) | Bias(1) | Relative error | Coefficient of variation | Precision | Resolution | rank |
---|---|---|---|---|---|---|---|
CqMAN | 2/2 | 1/1 | 3/1 | 1/2 | 1/1 | 4/2 | 2.08/1.58 |
Cy0 | 1/1 | 1/1 | 1/2 | 2/1 | 4/2 | 3/1 | 2.08/1.92 |
LinRegPCR | 3/6 | 2/4 | 4/4 | 4/3 | 3/3 | 1/4 | 3.00/3.67 |
PCR-Miner | 7/3 | 3/2 | 2/3 | 3/4 | 2/4 | 2/3 | 3.33/3.33 |
5PSM | 6/8 | 7/7 | 8/8 | 5/7 | 5/7 | 6/5 | 6.33/7.67 |
DART | 5/7 | 6/6 | 7/7 | 6/6 | 6/6 | 5/6 | 6.00/6.67 |
FPLM | 4/5 | 5/3 | 6/6 | 7/8 | 7/8 | 7/7 | 6.17/6.17 |
FPK-PCR | 8/4 | 4/5 | 5/5 | 8/5 | 8/5 | 8/8 | 7.00/5.00 |