Early life and adult stress promote sex dependent changes in hypothalamic miRNAs and environmental enrichment prevents stress-induced miRNA and gene expression changes in rats

McKibben, Lauren Allen; Dwivedi, Yogesh

doi:10.1186/s12864-021-08003-4

Table 2 miRNAs altered by the interaction between restraint stress, environmental enrichment, and sex in maternally separated rats

From: Early life and adult stress promote sex dependent changes in hypothalamic miRNAs and environmental enrichment prevents stress-induced miRNA and gene expression changes in rats

		CPM-Fold Change
Accession ID	miRNA					p	Sig.
restraint stress		RS:Control
MIMAT0004646	miR-338-5p	1.20				0.018	*
MIMAT0000587	miR-341	−1.13				0.043	*
enrichment		Enr:Non-Enr
MIMAT0017895	miR-3592	1.37				0.002	**
MIMAT0017094	miR-16-3p	1.30				0.002	**
MIMAT0017130	miR-144-5p	1.28				0.036	*
MIMAT0017158	miR-212-5p	1.28				0.022	*
MIMAT0017818	miR-3556b	1.27				0.001	***
MIMAT0017836	miR-9b-3p	1.26				0.022	*
MIMAT0005299	miR-181d-5p	1.26				0.002	**
MIMAT0000857	miR-181c-5p	1.25				0.007	*
MIMAT0017357	miR-362-3p	1.24				0.007	*
MIMAT0000820	miR-99a-5p	1.23				0.024	*
MIMAT0017850	miR-3570	1.22				0.015	*
MIMAT0017823	miR-3596d	1.22				0.010	*
MIMAT0005341	miR-488-3p	1.20				0.021	*
MIMAT0017360	miR-582-3p	1.20				0.029	*
MIMAT0017867	miR-3579	1.18				0.028	*
MIMAT0003207	miR-369-3p	1.17				0.005	*
MIMAT0005282	miR-872-5p	1.14				0.003	**
MIMAT0000867	miR-192-5p	1.14				0.027	*
MIMAT0004710	miR-17-1-3p	1.13				0.031	*
MIMAT0017031	miR-329-5p	1.13				0.009	*
MIMAT0003123	miR-377-3p	1.13				0.009	*
MIMAT0003204	miR-409a-5p	1.12				0.047	*
MIMAT0000553	miR-324-5p	−1.09				0.049	*
MIMAT0017371	miR-666-3p	−1.13				0.012	*
MIMAT0000794	miR-24-3p	−1.14				0.022	*
MIMAT0000562	let-7d-5p	−1.16				0.022	*
MIMAT0000784	miR-15b-5p	−1.16				0.020	*
MIMAT0035726	miR-149-5p	−1.17				0.029	*
MIMAT0004729	miR-125a-3p	− 1.18				0.035	*
MIMAT0017874	miR-3583-3p	−1.21				0.000	***
MIMAT0012845	miR-935	−1.21				0.028	*
MIMAT0017318	miR-873-3p	−1.22				0.031	*
MIMAT0000570	miR-331-3p	−1.23				0.022	*
MIMAT0000853	miR-150-5p	−1.26				0.033	*
MIMAT0001549	miR-365-3p	−1.27				0.004	**
MIMAT0001543	miR-449a-5p	−1.33				0.035	*
MIMAT0004742	miR-296-3p	−1.36				0.027	*
MIMAT0035752	miR-762	−1.37				0.029	*
MIMAT0000839, MIMAT0003126	miR-133a/b-3p	− 1.42				0.026	*
sex		Female:Male
MIMAT0035719	let-7 g-5p	1.15				0.030	*
MIMAT0000819	miR-98-5p	1.14				0.008	*
MIMAT0003154	miR-29c-5p	1.13				0.033	*
MIMAT0017175	miR-421-3p	1.12				0.002	**
MIMAT0005309	miR-384-5p	1.11				0.022	*
MIMAT0000796	miR-26a-5p	1.09				0.012	*
MIMAT0024846	miR-3068-3p	−1.07				0.023	*
MIMAT0005342	miR-652-3p	−1.10				0.015	*
MIMAT0005278	miR-466b-5p	−1.10				0.035	*
MIMAT0005311	miR-410-3p	−1.16				0.050	*
MIMAT0004715	miR-27a-5p	−1.18				0.027	*
MIMAT0000887	miR-217-5p	−1.23				0.039	*
MIMAT0025049	miR-344i	−1.25				0.008	*
MIMAT0012851	miR-547-3p	−2.51				0.000	***
RS x Enr		Enr:Non-Enr		Enr + RS:Non-Enr + RS
MIMAT0017043	miR-135b-3p	−1.04		1.27		0.022	*
RS x sex		RS Male:Control Male		RS Female:Control Female
MIMAT0017371	miR-666-3p	1.12		−1.09		0.043	*
Enr x sex		Enr Male:Non-Enr Male		Enr Female:Non-Enr Female
MIMAT0017887	miR-3588	1.45		−1.11		0.035	*
MIMAT0004705	let-7b-3p	1.23		−1.09		0.014	*
MIMAT0017877	miR-3596c	1.23		−1.12		0.049	*
MIMAT0000574	miR-140-3p	1.22		−1.18		0.036	*
MIMAT0000583	miR-339-5p	1.20		−1.17		0.016	*
MIMAT0003378	miR-378a-5p	1.16		−1.36		0.009	*
MIMAT0003213	miR-503-5p	1.15		−1.47		0.033	*
MIMAT0017109	miR-93-3p	1.13		−1.20		0.048	*
MIMAT0000830	miR-125b-5p	1.11		−1.21		0.028	*
MIMAT0004648	miR-339-3p	1.10		− 1.27		0.036	*
MIMAT0000608	miR-351-5p	1.10		−1.71		0.049	*
MIMAT0005299	miR-181d-5p	1.09		1.46		0.047	*
MIMAT0000792	miR-23a-3p	1.08		−1.24		0.045	*
MIMAT0004739	miR-204-3p	1.07		−1.75		0.034	*
MIMAT0017224	miR-503-3p	1.07		−1.54		0.048	*
MIMAT0025067	miR-6328	1.06		−1.34		0.043	*
MIMAT0005282	miR-872-5p	1.04		1.26		0.030	*
MIMAT0000774	let-7a-5p	1.01		−1.16		0.031	*
MIMAT0001549	miR-365-3p	−1.07		−1.48		0.038	*
MIMAT0005335	miR-758-3p	−1.07		1.22		0.017	*
MIMAT0005311	miR-410-3p	−1.08		1.36		0.016	*
MIMAT0025070	miR-6331	−1.11		1.36		0.012	*
MIMAT0004718	miR-29a-5p	−1.11		1.28		0.038	*
MIMAT0005287	miR-879-5p	−1.14		1.28		0.048	*
MIMAT0017136	miR-154-3p	− 1.15		1.70		0.024	*
MIMAT0017102	miR-31a-3p	−1.21		1.32		0.040	*
MIMAT0001628	miR-433-3p	−1.22		1.24		0.003	**
MIMAT0017111	miR-98-3p	−1.23		1.13		0.030	*
MIMAT0000568	miR-330-3p	−1.23		1.15		0.046	*
MIMAT0003381	miR-499-5p	−1.24		1.18		0.042	*
MIMAT0000611	miR-135b-5p	−1.25		1.30		0.020	*
MIMAT0005279	miR-466c-5p	−1.25		1.41		0.020	*
MIMAT0004791	miR-379-3p	−1.27		1.32		0.034	*
MIMAT0000813	miR-34b-5p	−1.27		1.38		0.014	*
MIMAT0000557	miR-325-5p	−1.28		1.35		0.017	*
MIMAT0017211	miR-540-5p	−1.29		1.28		0.011	*
MIMAT0000613	miR-151-5p	−1.30		1.25		0.043	*
MIMAT0000811	miR-32-5p	−1.31		1.28		0.038	*
MIMAT0000865	miR-190a-5p	−1.31		1.20		0.026	*
MIMAT0017104	miR-33-3p	−1.32		1.16		0.041	*
MIMAT0012833	miR-582-5p	−1.32		1.47		0.038	*
MIMAT0012834	miR-592	−1.33		1.38		0.022	*
MIMAT0000577	miR-337-3p	−1.33		1.29		0.041	*
MIMAT0017302	miR-380-3p	−1.34		1.32		0.034	*
MIMAT0024845	miR-3068-5p	−1.34		1.12		0.037	*
MIMAT0000787	miR-18a-5p	−1.35		1.28		0.023	*
MIMAT0017192	miR-433-5p	−1.39		1.27		0.011	*
MIMAT0000789	miR-19a-3p	−1.42		1.36		0.044	*
MIMAT0003195	miR-376b-5p	−1.43		1.21		0.048	*
MIMAT0000552	miR-301a-3p	−1.44		1.30		0.028	*
MIMAT0000825	miR-106b-5p	−1.45		1.23		0.049	*
MIMAT0000801	miR-29b-3p	−1.46		1.35		0.039	*
MIMAT0000855	miR-153-3p	−1.49		1.39		0.027	*
MIMAT0000842	miR-136-5p	−1.51		1.41		0.021	*
MIMAT0017135	miR-153-5p	−1.55		1.35		0.022	*
MIMAT0003176	miR-539-5p	−1.59		1.84		0.020	*
RS x Enr x sex		Enr Male: Non-Enr Male	Enr Female: Non-Enr Female	Enr + RS Male: Non-Enr RS Male	Enr RS Female: Non-Enr RS Female
MIMAT0005322	miR-532-5p	1.32	−1.03	−1.04	1.24	0.041	*
MIMAT0017090	let-7f-2-3p	1.27	1.02	−1.28	1.67	0.024	*
MIMAT0000827	miR-122-5p	1.21	−1.39	− 1.04	1.17	0.038	*
MIMAT0017139	miR-181b-1-3p	1.20	−1.18	−1.13	1.25	0.013	*
MIMAT0035748	miR-452-5p	1.18	−1.30	− 1.11	1.40	0.024	*
MIMAT0000803	miR-29c-3p	1.17	1.01	−1.14	1.37	0.048	*
MIMAT0004705	let-7b-3p	1.16	1.13	1.31	−1.33	0.025	*
MIMAT0000615	miR-101b-3p	1.12	−1.09	−1.15	1.31	0.040	*
MIMAT0012838	miR-653-5p	1.12	−1.11	− 1.12	1.49	0.026	*
MIMAT0012831	miR-544-3p	1.12	−1.12	−1.34	1.47	0.024	*
MIMAT0017143	miR-186-3p	1.09	−1.08	− 1.47	1.26	0.027	*
MIMAT0024846	miR-3068-3p	1.09	−1.03	−1.08	1.12	0.010	*
MIMAT0005282	miR-872-5p	1.08	1.10	−1.00	1.44	0.041	*
MIMAT0004711	miR-21-3p	1.06	−1.41	− 1.38	1.24	0.026	*
MIMAT0017877	miR-3596c	1.06	1.12	1.43	−1.40	0.020	*
MIMAT0017033	miR-331-5p	1.05	−1.10	−1.04	1.18	0.013	*
MIMAT0017871	miR-3596b	−1.01	1.15	1.34	−1.42	0.010	*
MIMAT0000791	miR-22-3p	−1.04	1.11	1.15	−1.35	0.023	*
MIMAT0000774	let-7a-5p	−1.07	−1.06	1.10	−1.26	0.029	*
MIMAT0000862	miR-185-5p	−1.12	−1.01	1.19	−1.31	0.019	*
MIMAT0000560	miR-326-3p	−1.14	1.04	1.13	−1.32	0.037	*
MIMAT0000810	miR-31a-5p	−1.16	1.05	1.26	−1.28	0.036	*
MIMAT0017802	miR-3547	−1.18	−1.01	1.31	−1.59	0.027	*
MIMAT0000562	let-7d-5p	−1.23	−1.08	1.08	−1.44	0.027	*
MIMAT0025067	miR-6328	−1.23	−1.19	1.44	−1.50	0.027	*
MIMAT0035726	miR-149-5p	−1.23	−1.09	1.07	−1.43	0.045	*
MIMAT0000554	miR-324-3p	−1.24	−1.09	1.33	−1.52	0.023	*
MIMAT0004641	miR-330-5p	−1.24	−1.00	1.07	−1.20	0.022	*
MIMAT0012844	miR-665	−1.30	−1.06	1.11	−1.36	0.039	*
MIMAT0012845	miR-935	−1.33	−1.18	1.18	−1.59	0.035	*
MIMAT0000853	miR-150-5p	−1.48	−1.08	1.15	−1.71	0.018	*
MIMAT0017885	miR-702-3p	−1.54	−1.11	1.24	−1.68	0.039	*

All animal subjects in this comparison received MS-180. miRNAs with a significant main effect or interaction effect are listed here. CPM-fold change is presented as a ratio one group’s miRNA expression over another group (usually the comparison control). Positive values indicate upregulation and negative values show downregulation. *p < 0.05, **p < 0.005, ***p < 0.001. Abbreviations: CPM-counts per million, MS-maternal separation, RS-restraint stress, Enr-environmental enrichment, C-control

Back to article page

ISSN: 1471-2164

Contact us

Submission enquiries: bmcgenomics@biomedcentral.com
General enquiries: ORSupport@springernature.com

BMC Genomics

Contact us