Table 3 Primers employed in the qPCR assay. Reference transcripts in the P. acuta transcriptome assembly are listed. *Primers for the ß tubulin are adopted from a previous study
| Functional annotation | Primer sequence (5’ – 3’) | Reference transcript |
|---|---|---|
| Angiotensin-converting enzyme |
f: gataaacagcagcgggaag r: agattcggtgacaaagacaag | TRINITY_DN5954_c0_g3_i1 |
|
f: actttctctgaaccccgac r: atccctccaccattccttcc | TRINITY_DN10767_c0_g1_i10 | |
|
f: caagtggatgatggaacagag r: agtgttgaacagtgtgggaag | TRINITY_DN3110_c4_g2_i4 | |
| Endothelin-converting enzyme |
f: cggaacatcaagcacagag r: aaaggacggtaatcaacacag | TRINITY_DN4184_c0_g1_i8 |
|
f: ctactcacccaggcaaaatc r: ccaatcaccataccaattccac | TRINITY_DN14970_c0_g1_i2 | |
|
f: tgactccccccactgtaaac r: ccaacaaccattccgattcc | TRINITY_DN36866_c0_g1_i3 | |
| Actin |
f: tgtctcgatcaataaaccttcc r: cccataccaaccatcactcc | TRINITY_DN21309_c1_g1_i1 |
| ß tubulin |
f: gcagttcacggctatgttc* r: ttttcaccctcctcttcctc* | Chuang and Mitarai (2020) |