Fig. 1

PCR-Restriction Digest Strategy to Resolve the TCR Gamma Locus. A schematic showing the TCR gamma locus according to Liu et al. 2020 and as depicted from galGal6a, with the latter appearing to have a tandem duplicated region. The PCR-restriction digest strategy to resolve this discrepancy is depicted including primer locations, Drdl restriction sites and expected product sizes (undigested product size in brackets). Regions of genomic DNA extracted from the spleens of 2 PA12 birds were amplified using three different primer sets and digested with the restriction enzyme Drdl and separated on a 1% agarose gel, visualised with ethidium bromide. Lanes 2–7 are with DrdI digestion, Lanes 8–13 are with no digestion. Product length was determined using a 100 bp NEB ladder. Ladder band lengths are indicated at either side of the gels in base pairs. Digested PCR products of expected sizes are indicated (white arrow) and support the Liu et al. 2020 [30] proposed structure for the TCR gamma locus