Fig. 6From: Repertoire analysis of γδ T cells in the chicken enables functional annotation of the genomic region revealing highly variable pan-tissue TCR gamma V gene usage as well as identifying public and private repertoiresTRGV Usage in Tissues of two Distinct lines of Chicken reveals Conserved Patterns of Expression. The proportional usage of each TRGV gene in productively (in-frame) rearranged TCR gamma is displayed in spleen and various intestinal tissues from ISA Brown and PA12 White Leghorn lines of chicken. Due to a high level of identity, some TRGV2 genes have been grouped. The TRGV genes on the X axis were all expressed in at least one tissue, pseudogenes and non-expressed genes are omitted. Bars represent the mean proportion for each TRGV gene displayed with 95% confidence intervals derived from 500 bootstrap replicates of an LMM using Bird number as a random variable and Tissue and TRGV gene as fixed variables. Proportions were logit transformed before model calculation. P values were derived from the LMM using lmerTest with the Satterthwaite approximation of degrees of freedom. (*** p value < 0.001, ** p value < 0.01, * p value < 0.05)Back to article page