Fig. 1
From: Impact of human gene annotations on RNA-seq differential expression analysis
Overview of the evaluation. In this study, we evaluated the impact of the complexity of gene annotation using experimental and simulated datasets. Overview of evaluation After a literature survey, we selected five major DE analysis pipelines: TopHat2-Cufflinks, STAR-RSEM-EBSeq, HISAT-StringTie-Ballgown, Kallisto-Sleuth, and Salmon-DESeq2 (see also “Materials and methods”). We used a benchmarking RNA-seq dataset established by the MicroArray Quality Control (MAQC) project as both the experimental dataset and simulation input