Control run (1 h) on total fecal DNA, no enrichment | Adaptive Sampling for 24 h, enriching to S. imperator scaffold genome | 2nd 24 h run enriching to S. imperator scaffold + L. chrysopygus mitogenome | ||||
---|---|---|---|---|---|---|
Total Reads | Alignments to L. chrysopygus mitogenome | Total Reads | Alignments to L. chrysopygus mitogenome | Total Reads | Alignments to L. chrysopygus mitogenome | |
Number of Sequences | 133,852 | 18 | 15,346,306 | 2810 | 13,362,519 | 2448 |
Total Length (bp) | 164,604,258 | 25,700 | 6,308,923,013 | 1,990,083 | 5,683,370,590 | 3,187,574 |
Average Length (bp) | 1230 | 1428 | 411 | 708 | 425 | 1302 |
Mb aligned to S. imperator | 649,354 | 50,352,101 | 46,619,227 | |||
% Aligned to S. imperator | 0.39% | 0.80% | 0.82% | |||
MtDNA hits per Mb | 0.11 | 0.45 | 0.43 | |||
mtDNA Mb / Total Mb | 0.016 | 0.032 | 0.056 | |||
mtDNA to host nuclear DNA | 3.96% | 3.95% | 6.84% |