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Table 1 Mapping statistics for Control and Adaptive Sampling Runs

From: The mitochondrial genome and Epigenome of the Golden lion Tamarin from fecal DNA using Nanopore adaptive sequencing

 

Control run (1 h) on total fecal DNA, no enrichment

Adaptive Sampling for 24 h, enriching to S. imperator scaffold genome

2nd 24 h run enriching to S. imperator scaffold + L. chrysopygus mitogenome

Total Reads

Alignments to L. chrysopygus mitogenome

Total Reads

Alignments to L. chrysopygus mitogenome

Total Reads

Alignments to L. chrysopygus mitogenome

Number of Sequences

133,852

18

15,346,306

2810

13,362,519

2448

Total Length (bp)

164,604,258

25,700

6,308,923,013

1,990,083

5,683,370,590

3,187,574

Average Length (bp)

1230

1428

411

708

425

1302

Mb aligned to S. imperator

649,354

 

50,352,101

 

46,619,227

 

% Aligned to S. imperator

0.39%

 

0.80%

 

0.82%

 

MtDNA hits per Mb

 

0.11

 

0.45

 

0.43

mtDNA Mb / Total Mb

 

0.016

 

0.032

 

0.056

mtDNA to host nuclear DNA

3.96%

 

3.95%

  

6.84%