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Table 6 Observed phenotypes by RNAi for the selected knock-down candidates

From: A novel approach to co-expression network analysis identifies modules and genes relevant for moulting and development in the Atlantic salmon louse (Lepeophtheirus salmonis)

TranscriptID

Gene annotation

Eggstring length

Cephalothorax length

Genital segment length

Other phenotypic changes

EMLSAT00000001458

Ras-related protein ORAB-1

no eggstrings

no significant difference to control lice

significantly shorter (29%) than control lice

abrogated transition copepodid to chalimus-1*

EMLSAT00000008959

Digestive organ expansion factor

no eggstrings

no significant difference to control lice

no significant difference to control lice

–

EMLSAT00000005299

ER membrane protein complex subunit 3

on AVG 20% shorter

no significant difference to control lice

slightly but significantly shorter (4%) than controls

–

EMLSAT00000003179

Cold shock domain-containing protein C2

no significant difference to control lice

slightly but significantly shorter (3%) than control lice

slightly but significantly shorter (7%) than control lice

–

EMLSAT00000004347

Hypothetical protein

on AVG 18% shorter

slightly but significantly longer (2%) than control lice

slightly but significantly shorter (6%) than control lice

weak cuticle of GS (easily squeezed and torn apart)

  1. All phenotypes were assessed in adult female lice after injection of dsRNA at the pre-adult 2 stage except for *: phenotypic change was observed at larval stages after dsRNA treatment of nauplii (see Fig. 4)