Skip to main content

Table 4 List of strains used in the present study. All listed strains are “mono-eukaryotic clonal cultures”, type of in vitro cultivation, xenic or monoxenic, is indicated. All H. meleagridis cultures were established in our laboratory from caecal material and faeces of infected turkeys or chickens that were sent to our Clinic (Clinic for Poultry and Fish Medicine, University of Veterinary Medicine, Vienna, Austria) for routine diagnostic investigation. The host bird species for each culture is indicated in the assignment of the strain according to following scheme: Histomonas meleagridis /bird species from which he parasite was isolated/country/diagnostic number – clone no. at micromanipulation/year of isolation

From: Complete genomes of the eukaryotic poultry parasite Histomonas meleagridis: linking sequence analysis with virulence / attenuation

strain

type of in vitro cultivation

passage

purpose

H. meleagridis/turkey/Austria/2922-C6/04/DH5αa

monoxenic

28p

Illumina and MINIon sequencing, confirmation of variants

346p

H. meleagridis/turkey/Austria/2922-C6/04b

xenic

13p

confirmation of variants

51p

83p

145p

237p

292p

H. meleagridis/chicken/Austria13250-C20/10c

xenic

24p

316p

H. meleagridis/turkey/Austria/2877-C3/05c

xenic

22p

H. meleagridis/chicken/Austria/8175-C7/06c

xenic

21p

  1. aassignment: Histomonas meleagridis isolated from turkey/country/diagnostic number – clone no. at micromanipulation/year of isolation/monoxenic grown with E. coli DH5α. Monoxenization was done independently at passage 10 (virulent) and 290 (attenuated) [13]
  2. bxenic cultures prior to monoxenization at passage 290
  3. c assignment Histomonas meleagridis /bird species from which he parasite was isolated/country/diagnostic number – clone no. at micromanipulation/year of isolation