Fig. 1
Significantly differentially enriched gene ontologies in mice associated with the expression of transgene-encoded antigens from an adenovirus vector. a Overlap between significantly differentially expressed genes (DEGs) in mouse whole blood 24 h after immunisation with human adenovirus serotype 5 (HuAd5) encoding a bacterial (factor H binding protein, Ad-fHbp), viral (pre-fusion F protein, Ad-preF), or no antigen (Ad-empty). Significantly DEGs were defined as those with an FDR < 0.01. Genes unique to each vaccine group represent putative transgene-encoded antigen-specific genes, while genes in the intersection between all three groups represent HuAd5 vector-specific genes. Gene ontology (GO) over-representation analysis was performed on significantly DEGs associated with (b) the Ad-fHbp vaccine and (c) the Ad-preF vaccine, defined relative to the Ad-empty group. The colour and position of circles along the x-axis corresponds to the negative log10 p-value associated with a given GO (y-axis), while the size of the circle corresponds to the number of genes defined for that GO. The horizontal grey line indicates the threshold for significant (p-value < 0.05)