Fig. 4

The PCR bias-corrected QuantSeq 3′ mRNA-Seq data in the degraded RNA (DV200 > 30%). A; Percentage of mapped reads out of total reads. Blue, non-PCR bias-corrected reads; Orange, PCR bias-corrected reads. B; Total number of detected genes. C; Similarity matrix in the paired FF and FFPE samples at 1 ng and 10 ng input. D; Correlation plot at 10 ng input between FF and FFPE samples. Samples 6-FF-70 and 8B-FFPE-70 are paired samples. Data were normalized by log₂ (TPM + 1). 6-FF-70, 70% of DV200; 8B-FFPE-70, 70% of DV200;; 2-FFPE-50, 50% of DV200;; 3-FFPE-40, 40% of DV200;; 2-FF-68, 68% of DV200. Each dot constitutes a gene