Fig. 2
From: Circadian miR-449c-5p regulates uterine Ca2+ transport during eggshell calcification in chickens
Identification of circadian miRNAs and functional analysis. A Heatmap of 734 miRNAs in chicken uterus at ZT4, ZT8, ZT12, ZT16, ZT20, and ZT0 (ZT24) time points. The value represents the log2 transformed values of (TPM + 1). B Heatmap of the six circadian miRNAs. C Six circadian miRNA-target gene correlation networks in the chicken uterus. Aqua green denotes ion transfer related genes. D Venn diagram showing the target genes of six circadian miRNAs using Targetscan and miRDB software, and uterine cyclical genes. E GO enrichment of the 127 circadian target genes. Top 15 terms of biological processes, cellular components, and molecular functions. The x-axis shows the number of genes. F KEGG [45] analysis showed the top 10 significantly enriched pathways associated with 127 circadian target genes