Fig. 5

ATP2B4 regulated Ca²⁺ transfer in the uterine tubular gland cells. A, B and C mRNA and protein expression of ATP2B4 was detected after transfection of the overexpression plasmid (pcDNA3.1-ATP2B4) and empty pcDNA3.1 vector. D, E, and F mRNA and protein expression of ATP2B4 was detected after transfection with small interfering RNA (Si-ATP2B4) and siRNA negative control (Si-NC). G and H The fluorescence intensity was measured using a microplate reader indicating the intracellular concentration of Ca²⁺ after overexpression and inhibition of miR-449c-5p. I and J Intracellular concentrations of Ca²⁺ ions were detected after transfection overexpression and inhibition of ATP2B4. Replications = 3. The samples were derived from the same experiment and the gels/blots were processed in parallel. Data are presented as mean ± standard error (SE); *P < 0.05 and **P < 0.01