Fig. 5

Three intramodular gene networks of differentially-expressed transcripts (DETs) of Frankliniella occidentalis larval guts in response to tomato spotted wilt virus infection during growth and development. Together, the 'turquoise' (A), 'blue' (B) and 'lightcyan' (C) modules accounted for ~ 80% of the DETs associated with virus infection. DETs associated with time-larval stages (3-L1, 24-L2, and 48-L2) are indicated. Transcripts that were in the top 10% of all module transcripts with regards to both module membership (kME, correlation of a node to a module eigengene) and intramodular connectivity (kIN, sum of connection weights between a node and all its network neighbors) were considered candidate hub transcripts (red shapes). Grey and pink lines indicate the correlations between transcripts and interconnections between transcripts and hubs, respectively. For better visualization of the interactions, a weight cutoff of ≥0.15 was applied for 'turquoise' and 'blue' networks, and a weight cutoff of ≥0.35 was applied for the 'lightcyan' network. Line thickness indicates strength of the connection. 3-L1 = cohort of first instars three hours after removal of virus inoculum after a 24-h acquisition access period; 24-L2 = same cohort of insects developed into early second instars, 24 h after removal of inoculum; and 48-L2 = same cohort of insects entering into late second instars, 48 h after removal of inoculum