Table 3 Hybrid assembly results for FSC237 isolate based on MinION data
Assembler | Short read library | Total length (bp) | GC (%) | Contigs (> = 500 bp) | NGA50 (Mb) | Genome covered (%) | Genomic features (Complete + partial) | Complete Busco (%) | True/all circular contigs (size) | Errors (per 100 kb) |
|---|---|---|---|---|---|---|---|---|---|---|
Canu/ Pilon | HiSeq | 1,949,612 | 32.26 | 1 | 1.95 | 99.97 | 3794 + 3 part | 93.24 | 1/1 (1891217) | 53.90 |
MiSeq | 1,942,673 | 32.34 | 1 | 1.94 | 99.97 | 3794 + 3 part | 62.16 | 0/0 | 351.18 | |
Ion Torrent | 1,948,850 | 32.27 | 1 | 1.95 | 99.97 | 3794 + 3 part | 88.51 | 1/1 (1890592) | 78.43 | |
Flye/Pilon | HiSeq | 1,921,160 | 31.96 | 3 | 1.89 | 99.97 | 3793 + 3 part | 77.70 | 1/1 (1893441) | 98.56 |
MiSeq | 1,944,710 | 31.58 | 3 | 1.92 | 99.97 | 3792 + 3 part | 64.86 | 1/1 (1913901) | 1059.83 | |
Ion Torrent | 1,921,860 | 31.95 | 3 | 1.89 | 99.97 | 3792 + 3 part | 67.57 | 0/0 | 162.61 | |
SPAdes | HiSeq | 1,892,530 | 32.28 | 1 | 1.86 | 98.21 | 3740 + 2 part | 93.92 | 1/1 (1891993) | 0.16 |
MiSeq | 1,827,899 | 32.33 | 32 | 0.09 | 96.58 | 3592 + 15 part | 93.92 | 0/0 | 20.84 | |
Ion Torrent | 1,858,435 | 32.28 | 2 | 1.40 | 98.19 | 3736 + 2 part | 91.89 | 0/1 (1498148) | 4.57 | |
Unicycler | HiSeq | 1,892,775 | 32.26 | 1 | 1.89 | 100.00 | 3794 + 3 part | 93.92 | 0/0 | 0.05 |
MiSeq | 1,921,618 | 32.25 | 12 | 1.89 | 99.88 | 3789 + 3 part | 93.92 | 1/1 (1891153) | 57.40 | |
Ion Torrent | 1,892,630 | 32.26 | 1 | 1.89 | 100.00 | 3794 + 3 part | 93.92 | 0/0 | 6.77 |