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Table 5 Donor constructs (ssODNS) designed for re-creating intragenic suppressors using homology-directed repair

From: Whole genome sequencing facilitates intragenic variant interpretation following modifier screening in C. elegans

Strain

ssODN Sequence (5′➔3′)

MTG309

TATATTGTTGAATTGGATACTCGTTGTCGGTTTGAGGTAGTAGCTCAAGGGAATTTCGTTAAACGAATTTTGATTG

MTG320

GGATTGTTCGTCAACGTAATCAATTCTTCTTCTCCTTTTCTTCCGCGTACTGTTCTATCAGGTATTCGGTGTACATAAACTGTTTGAACCATTTCATCAACTTCGACAATCAAAAT

MTG329

CTGGAATAACACTTACAAAAGTGAATGAAGTATATGAATATCTAATAAGATTTGAACAATGTCTTAACGGAATGGATCGAGGAATGGTGTG

MTG398

ATCTTATTTTTAGCGCACCAAGTGTTGATCAACTAAGAGATGAGCGGTGGGAAGAGTGGTTCAAGATTGAG

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BMC Genomics

ISSN: 1471-2164

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